The nigrosin-stain stain produces a dark background on which the sperm stand out as lightly colored objects. The technique for preparing an eosin-nigrosin-stained slide is as follows:. Collection and Evaluation of Semen.
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Collection and Evaluation of Semen. The technique for preparing an eosin-nigrosin-stained slide is as follows:. An nigrosin-eosin stain is commonly used because it is effective, simple and, in addition to allowing sperm to be readily visualized, it is a so-called "live-dead" stain, allowing one to assess membrane integrity at the same time as morphology.
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Pyriform pear-shaped sperm and bent, abnormal midpiece bull; eosin-nigrosin stain. Anatomic site of the defect: Dry the slide rapidly by placing in on a warming plate or waving it back and forth in the air. Pipet a drop of stain onto the end of a slide, then pipet a small droplet of semen next to the stain. Examine headed a bright field microscope typically using a X objective lens.
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